Electromyography (EMG) For Hamstring Screening

A Practical Use Case

Key Takeaways


1

Hamstring strains are common, recur often, and player unavailability is costly. Early, targeted insight helps keep athletes on the pitch.

2

Beyond jump and strength outputs, EMG shows which hamstrings are being recruited within each exercise, exposing asymmetries and compensations.

3

This hamstring screening revealed greater contribution from the right hamstrings and a left biceps femoris and left semitendinosus asymmetry, providing crucial information to guide programme design, exercise selection, and technique optimisation.

Hamstring Injuries – A Brief Overview

Hamstring injuries remain a major concern affecting athletes in running sports, accounting for approximately 10% of all injuries in field sports [1]. Within the hamstring group, the long head of the biceps femoris is the most frequently injured muscle [2, 3]. During sprinting, the hamstrings extend the hip and flex the knee, but in the late swing phase they shift to an eccentric role, decelerating the forward motion of the leg just before ground contact [4]. It is in this phase, when the muscles are both highly activated, contracting eccentrically and stretched to long lengths, that hamstring injuries typically occur.

Post-injury, changes in biomechanics are common as the neuromuscular system adapts sprint mechanics, such as shorter stride length and reduced hip extension, to protect the previously injured muscle [5]. While these adjustments allow the movement to be completed successfully, they often come at the expense of running efficiency and can increase the load on other hamstring and gluteal muscles. Over time, this compensatory strategy may elevate the risk of reinjury.

The tendency of hamstring injuries to recur, typically around 15-30% [6], with rates as high as 63% reported in some studies [7], is equally troubling. With the frequency and intensity of sport seemingly ever increasing, and player unavailability costly for clubs, the need for better tools to monitor, screen, and understand athletes’ neuromuscular function becomes more pressing. Electromyography (EMG) is one tool that can provide additional insights into this area by investigating these asymmetries, compensations and more.

How Can EMG Help in Applied Sport?

In applied sport, jump and strength tests are often used to monitor rehabilitation after injury. While these tests provide useful values, they can’t reveal how those results were achieved. Compensations following hamstring injury mean that similar performance values can be achieved through different activation strategies. EMG exposes these compensations, such as asymmetries in activation between medial and lateral hamstrings, which may increase reinjury risk. By combining EMG with other metrics, such as force, we can build a clearer picture of which muscles are producing actively recruited, whether asymmetries or inefficiencies are present, and ultimately, how these factors may influence injury or reinjury risk.

By measuring activity from the biceps femoris and semitendinosus on both legs, practitioners can see the relative contributions of each muscle during specific exercises. When used in this context, EMG helps practitioners use common exercises as a screening tool, revealing which muscles are most optimally recruited and what needs to change to allow an athlete to progress or reduce injury risk.

In this blog we examined muscle activation during six common hamstring exercises, using EMG to assess bilateral biceps femoris and semitendinosus contribution.

Data Collection

One healthy male participant completed a range of hamstring rehabilitation exercises. Four Trigno Avanti sensors were paired with a Trigno Lite receiver, and placed on:

  • Left Biceps Femoris
  • Left Semitendinosus
  • Right Biceps Femoris
  • Right Semitendinosus

Each location for sensor placement was shaved to remove any excess hair, cleaned with an alcohol wipe and then each Trigno Avanti sensor was placed following the SENIAM guidelines.

Normalisation

After placement, each sensor was normalised to a Maximum Voluntary Contraction (MVC). Normalisation is paramount when collecting surface EMG, to reduce the effect of the limitations of surface EMG, whilst providing a reference value to allow comparisons between muscles, athletes and sessions.

Hamstring Exercises

To assess the contribution of the hamstring muscles during different exercises, the following exercises were performed in a randomised order with 2 minutes rest between each exercise:

  • Nordic hamstring curl
  • Bridges (90° knee flexion)
  • Single leg bridge (90° knee flexion)
  • Long lever bridge (30° knee flexion)
  • Romanian deadlift (with 30kg barbell)
  • Roman chair (45° hip extension)

These exercises were selected due to their frequent usage in hamstring testing and rehabilitation programmes. EMG data was collected through Trigno Discover and all amplitudes normalised to the muscle specific MVC.

Note that dynamic exercises can exceed 100% of isometric MVC.

Results & Interpretation

Using the Trigno Analytics feature, reports were generated immediately after each exercise. Across the session, the right hamstrings typically showed higher activation levels than the left, reflected in both higher Average Max (%MVC) and a larger share of Total Activation. The standout finding was the under-recruitment of left BF across most tasks alongside comparatively high activity in the other muscles measured, illustrated clearly by the Amplitude reports for Bridges (Figure 1) and Romanian Deadlifts (Figure 2).

Symmetry reports further highlight the large asymmetry between left BF and left ST activation (Bridges – Figure 3), a common theme throughout the exercises. The only exception to this recruitment pattern was during Nordic Hamstring Curls. Because the left BF does reach high activation in the Nordics, this muscle may be under-recruited during hamstring exercises due to task or technique issues, raising some considerations and questions:

  1. Technique changes to bias BF – a first step may be to ensure technique is correct for these exercises and then alter the execution, for example with hip or foot rotation, to increase left BF activation.
  2. EMG guided biofeedback – during these technical changes, real time biofeedback can be used to help the athlete focus on left BF activation and visualise the effect of any alterations.
  3. Incorporate single leg exercises – asymmetry decreased during Single Leg Bridges (Figure 4). To help reinforce correct technique and reduce the input of the right leg, single leg exercises could be utilised more.
  1. Load distribution and risk – this asymmetry may be shifting strain towards the left ST or right hamstrings, as suggested by the Total Activation. This further evidences the need to bias left BF during exercises whilst monitoring this asymmetry.
  2. Transfer to sprinting – is the left BF activating correctly to be able to handle the high eccentric load that comes with sprinting? EMG could be used in a separate test to identify if a similar asymmetry between muscles is apparent during running and sprinting.
  3. EMG for exercise selection – with these findings, it is possible to assess which exercises most effectively recruit the targeted muscle(s) (Table 1). Read “Using EMG to Assist with Exercise Selection” for more information on how EMG can be used for this purpose.

Table 1 – Avg. Max EMG Amplitude (%MVC) for each exercise and each muscle, taken from the Trigno Analytics Amplitude Reports. All colours match those in the reports, with the lowest and highest activation for each muscle underlined and bold respectively.

Left Biceps Femoris Left Semitendinosus Right Biceps Femoris Right Semitendinosus
Bridges 14.01% 34.55% 59.72% 49.51%
Single Leg Bridges 35.05% 55.62% 76.64% 68.17%
Long Lever Bridges 13.87% 66.51% 56.57% 58.38%
Romanian Deadlift 29.11% 57.03% 54.13% 81.33%
Roman Chair 35.82% 93.69% 100.49% 113.24%
Nordic Hamstring Curls 139.78% 146.75% 152.85% 128.99%

Take Home Points

EMG adds an extra, crucial layer to athlete monitoring through hamstring screening. It shows how the hamstrings contribute, not just the performance outcome, allowing the right muscle to be targeted with the right exercise and verify changes. EMG complements strength and jump tests rather than replacing them, by giving practitioners the missing neuromuscular context behind those numbers.

  • What EMG shows: which hamstring muscles are most actively recruited within each exercise, exposing asymmetries and compensations
  • This athlete’s results: under-recruitment of the left biceps femoris in all tasks except Nordic Hamstring Curls and greater total activation on the right leg.
  • Potential next steps: incorporate more single leg exercises, bias left biceps femoris and refine technique, using EMG-guided biofeedback as a visual aid.
  • Why it matters: better information allows for better decisions with the goal of increased player availability and reduced costly days missed.
For more information on using EMG in hamstring screening in applied sports, please reach out to contact@delsyseurope.com.

References

  1. Maniar, N., Carmichael, D. S., Hickey, J. T., Timmins, R. G., San Jose, A. J., Dickson, J., & Opar, D. (2023). Incidence and prevalence of hamstring injuries in field-based team sports: a systematic review and meta-analysis of 5952 injuries from over 7 million exposure hours. British journal of sports medicine, 57(2), 109-116.
  2. Ekstrand, J., Healy, J. C., Waldén, M., Lee, J. C., English, B., & Hägglund, M. (2012). Hamstring muscle injuries in professional football: the correlation of MRI findings with return to play. British journal of sports medicine, 46(2), 112-117.
  3. Martin, R. L., Cibulka, M. T., Bolgla, L. A., Koc Jr, T. A., Loudon, J. K., Manske, R. C., … & Dauber, J. A. (2022). Hamstring strain injury in athletes: clinical practice guidelines linked to the international classification of functioning, disability and health from the academy of orthopaedic physical therapy and the American academy of sports physical therapy of the American physical therapy association. Journal of Orthopaedic & Sports Physical Therapy, 52(3), CPG1-CPG44.
  4. Schache, A.G., Dorn, T.W., Blanch, P.D., Brown, N.A. and Pandy, M.G., 2012. Mechanics of the human hamstring muscles during sprinting. Medicine & science in sports & exercise, 44(4), pp.647-658.
  5. Heiderscheit, B. C., Sherry, M. A., Silder, A., Chumanov, E. S., & Thelen, D. G. (2010). Hamstring strain injuries: recommendations for diagnosis, rehabilitation, and injury prevention. journal of orthopaedic & sports physical therapy, 40(2), 67-81.
  6. Abdulridha, K. H., Maseer, M. J., Cuenca-Zaldivar, J. N., Aguilar-Latorre, A., Calatayud, E., & Gómez-Soria, I. (2025). Comparative effectiveness of rehabilitation protocols for hamstring injuries: A systematic review and meta-analysis. Journal of bodywork and movement therapies.
  7. Pollock, N., Kelly, S., Lee, J., Stone, B., Giakoumis, M., Polglass, G., Brown, J., & MacDonald, B. (2022). A 4-year study of hamstring injury outcomes in elite track and field athletes using the British Athletics rehabilitation approach. British Journal of Sports Medicine, 56(5), 257–263

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